| Learning Outcomes |
PO |
MME |
| The students who succeeded in this course: |
|
|
| LO-1 |
Defines the basic concepts of plant preparation techniques. |
PO-1 Have an advanced level of knowledge about the recent and best practices in Biology, deepen and expand their knowledge on the expert level, conduct and assess the analyses by employing statistical methods
|
Examination
|
| LO-2 |
Discusses the classification of Fixation and Fixatives. |
PO-3 Make use of the knowledge they have in order to find a solution to problems by analyzing, comparing and questioning them and can carry out original research in the related field on their own
|
Examination
|
| LO-3 |
Painting, paints, dyes classification, mechanisms of painting, paints and chemical properties, describes the production of plant tissue dye. |
PO-9 Transfer in a systematical way the latest developments in Biology and their field of study reinforcing with qualitative and quantitative data to the groups either concerned with the field or not by verbal, visual and written means
|
Examination
|
| LO-4 |
Sectioning, staining, fixation, slide preparation techniques, evaluates permanent. |
PO-10 Monitor and supervise the social, scientific, cultural and ethical values in the process of collecting, interpreting, implementing and publishing the data related to the field of Biology and teach those values
|
Examination
|
PO: Programme Outcomes
MME:Method of measurement & Evaluation |
| Course Contents |
| General information
Report preparation and writing methods
Material collection techniques
The chemicals used for the determination of the material, properties, formulations and methods
Chemicals and solvents used to safeguard the protection of
Chemicals and methods used in the dehydration
Cross sections (by hand, with a microtome, microtome types, types of microtome knife)
Biological dyes, paint formulations, and methods
Temporary and fixed media review
Maceration methods, glycerin gelatin method
Paraffin method
types of Microscopy
Light microscopy types of light microscope, operating principles and components
drawing methods
shutdown preparations |
| Weekly Course Content |
| Week |
Subject |
Learning Activities and Teaching Methods |
| 1 |
Lenses, the lens of the chromatic defects, classification of the microscope, light microscopes |
Classroom lessons, question and answer |
| 2 |
Some portions of the light microscope-optics (lenses and types, and types of ocular, kondansör and types), the mechanical part (statif, trays, tubes, revolvers, macro and mikrovida) |
Classroom lessons, question and answer |
| 3 |
Stereo microscope, dark field microscope, phase-contrast microscopy, fluorescence polarization and microscopes, ultraviolet microscopy, X-ray microscope, electron microscopes (TEM, SEM |
Classroom lessons, question and answer |
| 4 |
Measurement and calibration of microscope (ocular and objective micrometers, calibration) |
Classroom lessons, question and answer |
| 5 |
Fixation and electron microscopy, stylish and Fixatives (aldehydes, Oxidizable Users, Users denatured protein, cross-connectors, physical factors, Microwave Fixation) |
Classroom lessons, question and answer |
| 6 |
Fixation for the selection of appropriate fixative (a mixture of single and Fixatives) and fixation of the factors affecting |
Classroom lessons, question and answer |
| 7 |
Fixatives reaction of some organic substances (nucleic acid, lipids, protein, carbohydrate, and enzyme reaction with biogenic amines); Fixatives used chemical substances |
Classroom lessons, question and answer |
| 8 |
mid-term exam |
|
| 9 |
Tissue tracking (water, waste disposal, transparent integration, impregnation, embedding in paraffin) |
Classroom lessons, question and answer |
| 10 |
Microtome types (rotary microtome, ultratom, piramidom, ice mikrotomu, sledge microtome) |
Classroom lessons, question and answer |
| 11 |
Taken from paraffin sections (sections adhering to the slide, sectioning errors) |
Classroom lessons, question and answer |
| 12 |
Staining of the sections (the general theory of paint, solvent-solvent interaction, marker-marker interaction, marker-tissue interaction |
Classroom lessons, question and answer |
| 13 |
Staining on the effects of different factors (fixation effect, the effect of tissue type) |
Classroom lessons, question and answer |
| 14 |
Natural dyes, mordant, metakromazi, paints nucleus, cytoplasm, paints, oil paints, stained preparations to close (closing media) |
Classroom lessons, question and answer |
| 15 |
Specific examination methods (tissue culture, autoradiography, freeze-drying, freeze-solving, ice-breaking techniques, the separation of cell fractions, immunositokimya, vital analysis methods) |
Classroom lessons, question and answer |
| 16 |
final exam |
|
| Recommend Course Book / Supplementary Book/Reading |
| 1 |
Algan G. Bitkisel Dokular İçin Mikroteknik, İstanbul, 1981. Ders Notları |
| Required Course instruments and materials |
| Algan G. Microtechnology for Plant Tissues, Istanbul, 1981.
Lecture Notes |
