Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

FACULTY OF LETTERS & SCIENCE / BIY 356 - BIOLOGY

Code: BIY 356 Course Title: MOLECULAR BIOLOGY LABORATORY Theoretical+Practice: 0+2 ECTS: 2
Year/Semester of Study 3 / Spring Semester
Level of Course 1st Cycle Degree Programme
Type of Course Compulsory
Department BIOLOGY
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer SEVİL SUNGUR (sevilsungur@nevsehir.edu.tr)
Name of Lecturer(s)
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
Molecular techniques to teach

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 Using molecular biological techniques to solve problems PO-1 Understand the basic concepts regarding the biology programs
PO-5 Are dedicated to improve themselves by keeping abreast of the developments in science, technology and modern topics
PO-9 Are able to express themselves in verbal and written communication having the ability to work individually, take initiatives and make their own decisions
PO-14 Are able to use the information and communication technology required by the field of Biology with a competence at the level of European Computer Driving License
Examination
LO-2 Knows moleculer techniques used at medicine and biotechnology PO-7 Are able to use the modern techniques and devices necessary for the current practices in the field of Biology
Examination
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
Kromosomal and Plasmid-DNA isolation of Bacteria and other microorganisms, Agarose gel electrophoresis Protein isolation SDS-PAGE, Transfer of genetic material in bacteria (transformation), Transfer of genetic material in bacteria (transformation),
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Kromosomal and Plasmid-DNA isolation of Bacteria and other microorganisms Experimentation
2 Agarose gel electrophoresis Experimentation
3 Protein isolation Experimentation
4 SDS-PAGE, Experimentation
5 Transfer of genetic material in bacteria (transformation), Experimentation
6 Transfer of genetic material in bacteria (transformation), Experimentation
7 Mutagens Implementation Experimentation
8 mid-term exam
9 Some bacteria and other microorganisms on Mutagenlerin Chemical Application Experimentation
10 Some bacteria and other microorganisms on Mutagenlerin Chemical Application Experimentation
11 Determination of Degree of Purity and quantity of DNA Experimentation
12 Polymerase chain reaction Experimentation
13 Polymerase chain reaction Experimentation
14 Enzymatic Restriction endonuclease cutting of DNA and Application of Enzymes Experimentation
15 Enzymatic Restriction endonuclease cutting of DNA and Application of Enzymes Experimentation
16 final exam
Recommend Course Book / Supplementary Book/Reading
Required Course instruments and materials
Freezer, autoclave, Electrophoresis System, vortex, gel imaging system (UVP), centrifuge device, laboratory consumables and chemicals.

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 2 40
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 2 60

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 2 14 28
Outside Class
       a) Reading 0
       b) Search in internet/Library 4 1 4
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 0
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 0
Own study for mid-term exam 3 2 6
mid-term exam 2 1 2
Own study for final exam 2 9 18
final exam 2 1 2
0
0
Total work load; 60