Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 3 / Spring Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Compulsory | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ENVER ERSOY ANDEDEN (enverandeden@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to teach the specific extraction and quantification of target molecules in various samples and the use of spectrophotometric techniques commonly used in the biochemistry laboratory and to teach the basic principles of these techniques. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain protein extraction and general purpose extraction techniques. |
PO-1 Obtain basic knowledge of Biology PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology |
Examination |
| LO-2 | Can apply spectrophotometric quantification, SDS-PAGE electrophoresis and purification techniques in proteins. |
PO-1 Obtain basic knowledge of Biology PO-2 Develop an understanding of matter and energy in organisms PO-3 Identify structure-function relationships in cells and organisms PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge |
Examination |
| LO-3 | Can explain the basic concepts of antioxidant activity determinations and spectrophotometric quantifications. |
PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-12 Demonstrate professional and ethical responsibility PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
| LO-4 | Can perform determination of several biomolecules. |
PO-3 Identify structure-function relationships in cells and organisms PO-7 Work effectively in multi-disciplinary research teams PO-8 Develop an awareness of continuous learning in relation with modern technology PO-9 Find out new methods to improve his/her knowledge PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-15 Have contributed to scientific advances with research and development abilities |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Total protein extraction from various numbers, quantitative quantification of proteins, SDS-PAGE electrophoresis, protein purification, methanol extraction from various samples, antioxidant activity assignment by DPPH method. | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Providing information about the experiments to be carried out during the period | Explanation |
| 2 | Total protein extraction | Explanation, Experimentation |
| 3 | Total protein extraction | Explanation, Experimentation |
| 4 | Spectrophotometric quantification of proteins | Explanation, Experimentation |
| 5 | Spectrophotometric quantification of proteins | Explanation, Experimentation |
| 6 | Introduction to SDS-PAGE electrophoresis | Explanation |
| 7 | SDS-PAGE electrophoresis | Explanation, Experimentation |
| 8 | mid-term exam | |
| 9 | SDS-PAGE electrophoresis | Explanation, Experimentation |
| 10 | Western Blotting technique-theoretical explanation | Explanation |
| 11 | Extraction for determination of antioxidant activity | Explanation, Experimentation |
| 12 | Determination of antioxidant activity | Explanation, Experimentation |
| 13 | Determination of antioxidant activity | Explanation, Experimentation |
| 14 | Analysis of results | Explanation |
| 15 | Overall rating | Explanation |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Nelson DL ve Cox MM, 2008. Lehninger Principles of Biochemistry (5. basım), WH Freeman | |
| 2 | Laboratuvar Föyü | |
| Required Course instruments and materials | ||
| Lecture notes, laboratory consumables and devices containing theoretical information and methodology regarding the experiment prepared by the faculty member teaching the course. | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 1 | 14 | 14 |
| b) Search in internet/Library | 1 | 14 | 14 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 7 | 14 |
| mid-term exam | 2 | 1 | 2 |
| Own study for final exam | 2 | 8 | 16 |
| final exam | 2 | 1 | 2 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||