Information Of Programmes

INSTITUTE OF SCIENCE / MBG505 - MONECULER BIOLOGY AND GENETICS

Code: MBG505

Course Title: BIOSEPERATION

Theoretical+Practice: 3+0

ECTS: 6

Year/Semester of Study

1 / Fall Semester

Level of Course

2nd Cycle Degree Programme

Type of Course

Optional

Department

MONECULER BIOLOGY AND GENETICS

Pre-requisities and Co-requisites

None

Mode of Delivery

Face to Face

Teaching Period

14 Weeks

Name of Lecturer

HİLAL İNCEBAY (hincebay@nevsehir.edu.tr)

Name of Lecturer(s)

Language of Instruction

Turkish

Work Placement(s)

None

Objectives of the Course

The aim is to teach the students the basic concepts of biodegradation techniques and their industrial applications from a chemistry point of view.

Learning Outcomes		PO	MME
The students who succeeded in this course:
LO-1	Can have information about academic studies or industrial / production applications, which are two areas in which biological separation methods can be taught in future professions by teaching on laboratory scale and industrial scale.	PO-11 Explains the basic functions of scientific processes, critically discusses and solves problems using appropriate statistical and computational methods, chooses contemporary experimental and theoretical approaches that will ultimately lead to the solution of molecular biology and genetic research problems	Examination Presentation
LO-2	Can learn the biological applications of separation processes, one of the important work areas of chemical engineering, and to be knowledgeable about the most important application fields of health and environment	PO-4 Being ethical and responsible individuals who are sensitive to social and universal values in the field of Molecular Biology and genetics, who look after the priority areas of the country, research and produce and contribute to these issues	Examination Presentation
LO-3	Can comprehend the modern structure of chemical engineering open to interdisciplinary interactions by recognizing biological processes	PO-9 Recognizes and explains the molecular biology and genetics of the problems of other disciplines	Examination Presentation
PO: Programme Outcomes MME:Method of measurement & Evaluation

Course Contents
Characteristics of biological systems. Separation methods in biological production processes. Extraction / enrichment of products in biological production processes by cell / protein / enzyme isolation, precipitation, centrifugation, extraction, adsorption, filtration, chromatography, crystallization, electrophoresis, reverse osmosis methods and membrane based methods. Comparison of laboratory scale biodegradation systems with industrial biodegradation systems. Examination and interpretation of the articles belonging to the original scientific studies related to the topic from the literature.
Weekly Course Content
Week	Subject	Learning Activities and Teaching Methods
1	General definitions of basic, purposeful and used methods of biosperation techniques	Lecture, discussion, question and answer
2	Characteristics of biological production systems, basic microbiology concepts	Lecture, discussion, question and answer
3	Usage of biological systems in industrial production processes: current use areas (dairy products, wine, agriculture, textile, medicine, waste water cleaning etc.) and environmentally friendly potential use areas (bioethanol, biodiesel, biopolymers etc.)	Lecture, discussion, question and answer
4	Cell / protein / enzyme isolation techniques: mechanical, chemical and biological methods	Lecture, discussion, question and answer
5	Separation by sedimentation: organic solvents, salts and precipitation mechanism Separation by centrifugation: Laboratory scale, preparative scale and ultrafiltration	Lecture, discussion, question and answer
6	Separation by extraction: solvent systems, aqueous two phase separation technique, continuous and continuous systems, supercritical extraction method	Lecture, discussion, question and answer
7	Separation by adsorption: Adsorbents, separation mechanisms, intermittent and packed bed separation systems	Lecture, discussion, question and answer
8	mid-term exam
9	Filtrasyon ile ayırma: Kullanılan ekipmanlar, ayırma mekanizması	Lecture, discussion, question and answer
10	Chromatographic separation: Liquid and gas chromatography methods, column systems, TLC, HPLC, FPLC, gel filtration, ion exchange, hydrophobic chromatography techniques	Lecture, discussion, question and answer
11	Separation by crystallization	Lecture, discussion, question and answer
12	Separation by electrophoresis: Gel and free flow techniques	Lecture, discussion, question and answer
13	Membrane separation techniques: Equipment used, dialysis, membrane chromatography, reverse osmosis	Lecture, discussion, question and answer
14	Oral presentations, discussions	Lecture, discussion, question and answer
15	Oral presentations, discussions	Lecture, discussion, question and answer
16	final exam
Recommend Course Book / Supplementary Book/Reading
1	1. Ghosh, R., Principles of Bioseparations Engineering, World Scientific Publishing, Singapore¬, 2006. YARDIMCI KİTAPLAR: 1. Desai, M.A., Downstream Processing of Proteins, Humana Press, Totowa, New Jersey, 2000.
2	2. Sham, H., Biotechnology, 2. Basım, Sayı 1: Biological Fundamentals, VCH, Almanya, 1993.
Required Course instruments and materials
projection

Assessment Methods
Type of Assessment	Week	Hours	Weight(%)
mid-term exam	8	2	40
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam	16	2	60

Student Work Load
Type of Work	Weekly Hours	Number of Weeks	Work Load
Weekly Course Hours (Theoretical+Practice)	3	14	42
Outside Class
a) Reading	2	12	24
b) Search in internet/Library	3	12	36
c) Performance Project			0
d) Prepare a workshop/Presentation/Report	4	10	40
e) Term paper/Project			0
Oral Examination			0
Quiz			0
Laboratory exam			0
Own study for mid-term exam	3	6	18
mid-term exam	1	1	1
Own study for final exam	3	7	21
final exam	1	1	1
			0
			0
Total work load;			183