Nevşehir Hacı Bektaş Veli University Course Catalogue

Information Of Programmes

INSTITUTE OF SCIENCE / KİM622 - TEKLİF EDİLEN KIMYA (DOKTORA)

Code: KİM622 Course Title: MOLECULAR ANALYSIS OF PROTEINS Theoretical+Practice: 3+0 ECTS: 6
Year/Semester of Study 1 / Spring Semester
Level of Course 3rd Cycle Degree Programme
Type of Course Optional
Department TEKLİF EDİLEN KIMYA (DOKTORA)
Pre-requisities and Co-requisites None
Mode of Delivery Face to Face
Teaching Period 14 Weeks
Name of Lecturer ŞEYDA ÇİĞDEM ÖZKAN (seydaozkan@nevsehir.edu.tr)
Name of Lecturer(s)
Language of Instruction Turkish
Work Placement(s) None
Objectives of the Course
The aim of this course is to teach which techniques are used for molecular analysis of proteins and how these techniques are used in practice.

Learning Outcomes PO MME
The students who succeeded in this course:
LO-1 Can define proteins and their properties. PO-1 Develop advanced theoretical and applied knowledge in the field, depending on undergraduate and graduate qualifications.
 		Examination
LO-2 Can explain the protein analysis methods. PO-1 Develop advanced theoretical and applied knowledge in the field, depending on undergraduate and graduate qualifications.
 		Examination
LO-3 Can have information about how the protein analysis methods applied today are done. PO-1 Develop advanced theoretical and applied knowledge in the field, depending on undergraduate and graduate qualifications.
PO-5 Can have the ability to search literature, make presentations, design and prepare experimental setups, and interpret the relevant results.
PO-15 Have adequate knowledge of computer software used in chemistry and use information and communication technologies at an advanced level.
 		Examination
Presentation
PO: Programme Outcomes
MME:Method of measurement & Evaluation

Course Contents
As part of the course content, the definition of proteins, their properties, structures, protein analysis methods, determination of molecular weights, sequence analysis of proteins, and practical applications will be explained.
Weekly Course Content
Week Subject Learning Activities and Teaching Methods
1 Definition of proteins, explanation of their functions and properties Lecture, Discussion, Question and Answer
2 Amino acids, properties of amino acids, peptide bond and properties, Lecture, Discussion, Question and Answer
3 Structure, classification and denaturation of proteins Lecture, Discussion, Question and Answer
4 Introduction to Protein Analysis Methods: Analysis Methods Based on Molecular Size Lecture, Discussion, Question and Answer
5 Introduction to Protein Analysis Methods: Analysis Methods Based on Differences in Solubility Lecture, Discussion, Question and Answer
6 Introduction to Protein Analysis Methods: Analysis Methods Based on Electrical Charge Lecture, Discussion, Question and Answer
7 Introduction to Protein Analysis Methods: Analysis Methods Based on Selective Adsorption and Specific Ligands Lecture, Discussion, Question and Answer
8 mid-term exam
9 Determination of Molecular Weights of Proteins: Gel Filtration Chromatography and SDS-PAGE Lecture, Discussion, Question and Answer
10 Protein Analysis Methods: Blotting Techniques (Western-Eastern Blot) Protein Sequence Analysis Lecture, Discussion, Question and Answer
11 Presentation of Articles Using Protein Analysis Methods Source Search, Presentation
12 Presentation of Articles Using Protein Analysis Methods Source Search, Presentation
13 Presentation of Articles Using Protein Analysis Methods Source Search, Presentation
14 Presentation of Articles Using Protein Analysis Methods Source Search, Presentation
15 Presentation of Articles Using Protein Analysis Methods Source Search, Presentation
16 final exam
Recommend Course Book / Supplementary Book/Reading
1 Keha, E. E., Küfrevioğlu, Ö. İ. (2012). Biyokimya, Aktif Yayınevi.
2 Dennison, C.. Protein Saflaştırma Rehberi, Aktif Yayınları.
Required Course instruments and materials
Computer, projection.

Assessment Methods
Type of Assessment Week Hours Weight(%)
mid-term exam 8 2 30
Other assessment methods
1.Oral Examination
2.Quiz
3.Laboratory exam
4.Presentation 12 2 10
5.Report
6.Workshop
7.Performance Project
8.Term Paper
9.Project
final exam 16 2 60

Student Work Load
Type of Work Weekly Hours Number of Weeks Work Load
Weekly Course Hours (Theoretical+Practice) 3 14 42
Outside Class
       a) Reading 3 11 33
       b) Search in internet/Library 3 12 36
       c) Performance Project 0
       d) Prepare a workshop/Presentation/Report 3 12 36
       e) Term paper/Project 0
Oral Examination 0
Quiz 0
Laboratory exam 0
Own study for mid-term exam 2 5 10
mid-term exam 1 1 1
Own study for final exam 3 7 21
final exam 1 1 1
0
0
Total work load; 180