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| Year/Semester of Study | 4 / Spring Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | MOLECULAR BIOLOGY AND GENETICS | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | ENVER ERSOY ANDEDEN (enverandeden@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to provide students with information about new and promising molecular techniques that are current in molecular biology and have been discovered in recent years. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain the basic concepts of molecular cloning and recombinant protein production. |
PO-1 Obtain basic knowledge of Biology PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
| LO-2 | Can explain the basic principles of genome editing technologies. |
PO-1 Obtain basic knowledge of Biology PO-2 Develop an understanding of matter and energy in organisms PO-13 Obtain knowledge about current issues in the field of molecular biology and genetics PO-14 Have knowledge about the main research interests of the molecular biology and genetics |
Examination |
| LO-3 | Can explain the importance of recombinant proteins and the uses of recombinant microorganisms in industry. |
PO-1 Obtain basic knowledge of Biology PO-11 Apply biological concepts to personal, social, economical, technological and ethical issues PO-14 Have knowledge about the main research interests of the molecular biology and genetics PO-17 Survey, analyze and interpret the experimental results and to reach a scientific result using the available scientific data. |
Examination |
| LO-4 | Can explain the uses of some nucleic acid types in diagnosis and treatment. |
PO-8 Develop an awareness of continuous learning in relation with modern technology PO-22 To know molecular techniques and their usage areas. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Molecular cloning, obtaining recombinant organisms, recombinant protein production in eukaryotic and prokaryotic hosts, CRISPR-cas9 and other genome editing technologies, DNA sequencing technologies, aptamers, molecules used in molecular diagnosis | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Introduction to molecular cloning | Oral expression |
| 2 | Molecular cloning techniques | Oral expression |
| 3 | Genome editing technologies | Oral expression |
| 4 | CRISPR-Cas9 | Oral expression |
| 5 | Recombinant protein production | Oral expression |
| 6 | Recombinant protein production (Prokaryotic host) | Oral expression |
| 7 | Recombinant protein production (Eukaryotic host) | Oral expression |
| 8 | mid-term exam | |
| 9 | Nucleic acids as therapeutic agents | Oral expression |
| 10 | Nucleic acids as therapeutic agents | Oral expression |
| 11 | Molecular diagnosis | Oral expression |
| 12 | Use of recombinant microorganisms in the industrial field | Oral expression |
| 13 | Use of recombinant microorganisms in the industrial field | Oral expression |
| 14 | DNA sequencing technologies | Oral expression |
| 15 | A general review of the lessons | Oral expression |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th Edition Bernard R. Glick, Cheryl L. Patten | |
| Required Course instruments and materials | ||
| Molecular Biotechnology; Author Name: Bernard R. Glick; Publisher: Palme Publishing House-Akademik; Number of Pages: 738 ; ISBN: 9786052826232 | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 30 |
| Other assessment methods | |||
| 1.Oral Examination | 6 | 2 | 5 |
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | 9 | 2 | 5 |
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 2 | 14 | 28 |
| Outside Class | |||
| a) Reading | 2 | 6 | 12 |
| b) Search in internet/Library | 3 | 6 | 18 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 2 | 5 | 10 |
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 2 | 5 | 10 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 2 | 5 | 10 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 90 | ||