Nevşehir Hacı Bektaş Veli University Course Catalogue
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| Year/Semester of Study | 1 / Fall Semester | ||||
| Level of Course | 3rd Cycle Degree Programme | ||||
| Type of Course | Optional | ||||
| Department | TEKLİF EDİLEN KIMYA (DOKTORA) | ||||
| Pre-requisities and Co-requisites | None | ||||
| Mode of Delivery | Face to Face | ||||
| Teaching Period | 14 Weeks | ||||
| Name of Lecturer | BAHAR TUBA FINDIK (btfindik@nevsehir.edu.tr) | ||||
| Name of Lecturer(s) | |||||
| Language of Instruction | Turkish | ||||
| Work Placement(s) | None | ||||
| Objectives of the Course | |||||
| The aim of this course is to learn the necessary methods for the purification and identification of proteins and to provide the theoretical knowledge to follow current publications in the fields of recombinant protein technology, biotechnology, and pharmacology. | |||||
| Learning Outcomes | PO | MME | |
| The students who succeeded in this course: | |||
| LO-1 | Can explain the basic methods used in the separation and purification of proteins. |
PO-1 Develop advanced theoretical and applied knowledge in the field, depending on undergraduate and graduate qualifications. PO-4 Can systematically follow up-to-date information in the field. PO-7 Can develop new ideas and methods with different perspectives by using high-level competencies such as suggesting solutions to problems that arise in the field and making decisions. |
Examination Performance Project |
| LO-2 | Can follow the innovations in protein purification and develop specific methods. |
PO-2 Can make connections between different disciplines and sub-fields that their field is related to by using their expert knowledge and skills, work in harmony in research groups. PO-4 Can systematically follow up-to-date information in the field. PO-8 Can design theoretical, experimental, or modeling-based research that will contribute to progress in the field, develop new scientific methods, and apply a known method to a different field. |
Examination |
| LO-3 | learn in-depth details about how protein purification works. |
PO-4 Can systematically follow up-to-date information in the field. PO-7 Can develop new ideas and methods with different perspectives by using high-level competencies such as suggesting solutions to problems that arise in the field and making decisions. |
Examination |
| PO: Programme Outcomes MME:Method of measurement & Evaluation |
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| Course Contents | ||
| Protein overview, purpose of protein purification and pre-planning, pre-separation techniques, centrifugation, introduction to chromatography, basic principles of chromatography, column chromatography, ion exchange chromatography, gel filtration chromatography, affinity chromatography, normal phase and reversed phase chromatography, electrophoretic methods, purity control and characterization, and recombinant protein technology subjects constitute the course content. | ||
| Weekly Course Content | ||
| Week | Subject | Learning Activities and Teaching Methods |
| 1 | Overview of proteins | Lecture, question and answer, discussion |
| 2 | Purpose of Protein Purification and Pre-Planning | Lecture, question and answer, discussion |
| 3 | Pre-Separation techniques | Lecture, question and answer, discussion |
| 4 | Pre-Separation techniques (Continued) | Lecture, question and answer, discussion |
| 5 | Centrifugation | Lecture, question and answer, discussion |
| 6 | Introduction to chromatography, The theory of chromatography | Lecture, question and answer, discussion |
| 7 | Column chromatography | Lecture, question and answer, discussion |
| 8 | mid-term exam | |
| 9 | Ion exchange chromatography | Lecture, question and answer, discussion |
| 10 | Gel Filtration Chromatography | Lecture, question and answer, discussion |
| 11 | Affinity Chromatography | Lecture, question and answer, discussion |
| 12 | Reverse Phase/Normal Phase Chromatography | Lecture, question and answer, discussion |
| 13 | Electrophoretic Methods | Lecture, question and answer, discussion |
| 14 | Purity Control and Characterization | Lecture, question and answer, discussion |
| 15 | Recombinant Protein Technology | Lecture, question and answer, discussion |
| 16 | final exam | |
| Recommend Course Book / Supplementary Book/Reading | ||
| 1 | Nelson D. L., Cox M. M., Lehninger (Kılıç, N. (Ed.)) (2005). Biyokimyanın İlkeleri, 3. Baskı. Ankara: Palme yayıncılık. | |
| 2 | Voet, D. and Voet, J.G. (1995). Biochemistry, 2nd edition, J. Wiley & Sons, N.Y.. | |
| Required Course instruments and materials | ||
| Lecture notes, books, related articles, computer | ||
| Assessment Methods | |||
| Type of Assessment | Week | Hours | Weight(%) |
| mid-term exam | 8 | 2 | 40 |
| Other assessment methods | |||
| 1.Oral Examination | |||
| 2.Quiz | |||
| 3.Laboratory exam | |||
| 4.Presentation | |||
| 5.Report | |||
| 6.Workshop | |||
| 7.Performance Project | |||
| 8.Term Paper | |||
| 9.Project | |||
| final exam | 16 | 2 | 60 |
| Student Work Load | |||
| Type of Work | Weekly Hours | Number of Weeks | Work Load |
| Weekly Course Hours (Theoretical+Practice) | 3 | 14 | 42 |
| Outside Class | |||
| a) Reading | 4 | 8 | 32 |
| b) Search in internet/Library | 4 | 8 | 32 |
| c) Performance Project | 0 | ||
| d) Prepare a workshop/Presentation/Report | 0 | ||
| e) Term paper/Project | 0 | ||
| Oral Examination | 0 | ||
| Quiz | 0 | ||
| Laboratory exam | 0 | ||
| Own study for mid-term exam | 4 | 8 | 32 |
| mid-term exam | 1 | 1 | 1 |
| Own study for final exam | 5 | 8 | 40 |
| final exam | 1 | 1 | 1 |
| 0 | |||
| 0 | |||
| Total work load; | 180 | ||